Elicitation effect on cell biomass and production of alkaloids in cell suspension culture of the tropical tree Eurycoma longifolia

Elicitation effect on cell biomass and production of alkaloids in cell suspension culture of the tropical tree Eurycoma longifolia

Authors

  • Chan Lai Keng
  • Ang Sze Wei
  • Arvind Bhatt

DOI:

https://doi.org/10.22458/urj.v2i2.160

Keywords:

Cell suspension culture, Eurycoma longifolia, medicinal plant, secondary metabolites.

Abstract

The present study tests the effect of different elicitor on cell biomass and alkaloid production in Eurycoma longifolia cells. The cell suspension cultures were prepared by inoculating 0,5g FW (fresh weight) of cells obtained from first batch of suspension cultures prepared from callus in liquid MSBs medium. Different concentration of chitosan, NaH2PO4, Na2CO3 and polyvinylpyrrolidone (PVP) were added as elicitor to optimize cell biomass and alkaloid production. MSBs medium supplemented with100mg/L chitosan induced significant increment in the cell biomass while higher amount of chitosan (150mg/L) induced the highest production of 9-hydroxycanthin-6-one (0,44%) but not 9-methoxycanthin-6-one. The addition of 2mg/L and 20mg/L NaH2PO4 induced the highest increment in cell biomass and alkaloid production respectively. However, addition of different concentration Na2CO3 and polyvinylpyrrolidone showed inhibitory effect on cell growth with no significantly increased in alkaloid production.

References

Bedir, E., J. Abou-Gazar, N. Ngwendson & I.A. Khan . 2003. Eurycomaoside: A Novel Compound from the Roots of Eurycoma longifolia. Chemical Pharmaceutical Bulletin 51: 1301-1303.

Brodelius, P., MA. Collinge, C. Funk, K. Gügler & I. Marques. 1989. Studies on alkaloid formation in plant cell cultures after treatment with a yeast elicitor. In: W.G.W. Kurz (ed.), Pri¬mary and secondary metabolism of plant cell cultures Springer-Verlag, Berlin.

Buitelaar, R.M., M.T. Cesario & J. Tramper. 1992. Elicitation of thiophene production by hairy roots of Tagetes patula. Enzyme Microbial Technology 14: 2–7.

Chan, K., C.Y. Choo & N. Abdullah. 2005. Semisynthetic 15-O-acyl- and 1,15-di-O-acyleurycomanones from Eurycoma longi¬folia as potential antimalarials. Planta Medica 71: 967-969.

Ciddi, V., V. Srinivasan & M.L. Shuler. 1995. Elicitation of Taxus sp. cell culture for production of taxol. Biotechnology Letters 17:1343–1346.

Goh, S.H., C.H. Chuah, J.S.L. Mok & E. Soepadmo. 1995. Malaysian Medicinal Plants for the Treatment of Cardiovascular Di¬seases, Pelanduk Publication Sdn. Bhd., Selangor, Malaysia.

Hout, S., A. Chea, S. Bun, R. Elias, M. Gasquet, P. Timon-David, G. Balansard & N. Azas . 2006. Screening of selected indige¬nous plants of Cambodia for antiplasmodial ativity. Jour¬nal Ethnopharmacology 107:12-18.

Jiwajinda, S., V. Santisopasri, A. Murakami, M. Kawanaka, H. Kawa¬naka, M. Gasquet, R. Eilas, G. Balansard & H. Ohigashi. 2002. In vitro anti-tumor promoting anti-parasitic activities of the quassinoids from Eurycoma longifolia, a medicinal plant in Southeast Asia. Journal Ethnopharmacology 82: 55-58.

Kuo, P., L. Shi, A. Damu, C. Su, C.Huang, C. Ke, J. Wu, A. Lin, K. Bas¬tow, K. Lee & T. Wu. 2003. Cytotoxic and antimalarial beta-carboline alkaloids from the roots of Eurycoma longifolia. Journal Natural Products 66: 1324-1327.

Lufthi, A. 2004. Pengoptimuman kultur ampaian sel eurycoma longifolia jack untuk penghasilan biojisim dan alkaloid. Ph.D. Thesis. Universiti Sains Malaysia. Penang, Malaysia.

Morita, H., E. Kishi, K. Takeya, H. Itokawa & O. Tanaka. 1990. New quassinoids from the roots Eurycoma longifolia. Chemistry Letters 5: 749-752.

Nurhanan, M.Y., L. Azimahtol, A. Mohd & M. Mohd. 2005. Cytotoxic effects of the root extracts of Eurycoma longifolia Jack. Phytotherapy Research 19: 994-996.

Osman, A., B. Jordan, P. Lessard, N. Muhammad, M. Haron, N. Riffin, A. Sinskey, C. Rha & D. Housman. 2003. Genetic diversity of Eurycoma longifolia inferred from single nucleotide polymorphisms. Plant Physiolology 131: 1294-1301.

Palazón, J., R. Cusido, M. Bonfil, A. Mallol, E. Moyamo, C. Mara¬les & M. Pinol. 2003. Elicitation of different Panax ginseng transformed root phenotypes for an improvement ginsenoside production. Plant Physiology Biochemistry 41: 1019-1025.

Perry, L. & J. Metzger. 1980. Medicinal plants of East and Southeast Asia: Attributed properties and uses. M.I.T., Cambridge, United Kingdom

Tee, T.T. & H. Azimzhtol. 2005. Induction of apoptosis by Eurycoma longifolia Jack extracts. Anticancer Research 25: 2205-2213.

Toivonen, L., M. Ojala & V. Kauppinen. 1991. Studies on the optimization of growth and indole alkaloids production by hairy roots cultures of Catharanthus roseus. Biotechnolology Bioengineering 37: 673-680.

Ueda, J.Y., Y. Tezuka, A. Banskota, Q. Le-Tran, Q. Tran, Y. Harimaya, I. Saiki & S. Kadota. 2002. Antiproliferative activity of Vietnamese medicinal plants. Biological & Pharmaceutical Bulletin 25: 735-760.

Vázquez–Flota, F., O. Moreno-Valenzuela, M. Miranda-Ham, J. Co¬ello-Coello & V. Loyola-Vargas.1994. Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures. Medium optimization and elicitation. Plant Cell, Tissue Organ Culture. 38: 273–279.

Zhang, C., X. Mei, L. Yu & X. Liu. 2000. Initiation, growth and taxol production of Taxus chinensis embryo-derived cell line. Journal Huazhong University of Science Technolo¬gy 28: 60–63.

Zhao, J., T. Lawrence, C. Davis & R. Verpoorte. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances. 23: 283–333

Zhao, J., Z. Wei-Hua, H. Qiu & G. Ying-Qing. 2000. Improvement of indole alkaloid production in Catharanthus roseus cell. cultures by osmotic shock. Biotechnology Letters 22: 1227–1231.

Zhari, I., I. Norhayati & L. Jaafar. 1999. Malaysian Herbal Monograph. National Pharmaceutical Control Bureau, Ministry of Agriculture, Malaysia.

Zhong, J.J. 2001. Biochemical engineering of the production of plant-specific secondary metabolites by cell suspension cultures. Advances in Biochemical Engineering/Biotechnology 72:26.

Vol 2. No 2 (2010)

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Published

2010-12-01

How to Cite

Keng, C. L., Wei, A. S., & Bhatt, A. (2010). Elicitation effect on cell biomass and production of alkaloids in cell suspension culture of the tropical tree Eurycoma longifolia. UNED Research Journal, 2(2), 239–244. https://doi.org/10.22458/urj.v2i2.160

Issue

Vol. 2 No. 2 (2010)

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