Macrocytic-normochromic anaemia in the African catfish Clarias gariepinus (Siluriform: Clariidae) exposed to Paraquat under laboratory conditions

Macrocytic-normochromic anaemia in the African catfish Clarias gariepinus (Siluriform: Clariidae) exposed to Paraquat under laboratory conditions




Toxicity, herbicides, stun, haematology, disorder, poisoning


Introduction: Agricultural pesticides are among the main causes of pollution in aquatic ecosystems, and they can lead to physiological changes in fish. For example, blood alteration and damage to haemopoietic tissue can be associated with pathological conditions related to water. Objective: To describe the effects of certain levels of pesticide on a Nigerian fish species. Methods: Macrocytic-normochromic anaemia was induced in C. gariepinus at intervals of 1,7 and 14 days (sub-lethal concentrations of Paraquat: 0; 0,03; 0,05; 0,07 and 0,09mg/l). Results: Blood dyscrasias was observed with a significant (p<0,05) decrease in haemoglobin, haematocrit, red blood cells, white blood cells, lymphocytes and monocytes. Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH), Neutrophils, Eosinophil and Basophil increased significantly (p<0,05) with increasing concentrations of the toxicant while Mean corpuscular haemoglobin concentration (MCHC) remained normal. Conclusion: Sublethal concentrations of paraquat induced macrocytic-normochromic anaemia in the catfish C. gariepinus.


Abubakar, M. I. (2013). Toxicity of 2, 3-dichlorovinyl dimethyl phosphate (Sniper 1000EC) on C. gariepinus (Burchell, 1822) and Oreochromis niloticus (Trewavas, 1983) under laboratory conditions. [Unpublished Ph.D Thesis, Federal University of Agriculture].

Abubakar, M.I, & Abdulsalami, S.A. (2013). Induction of Microcytic-Hypochromic Anaemia in C. gariepinus (Burchell, 1822) Exposed to Sublethal Toxicity of 2, 3-Dichlorovinyl Dimethyl Phosphate (Sniper 1000EC) Under Laboratory Conditions. Journal of Medical and Applied Biosciences, 5(2), 62 – 72.

Abubakar, M.I. (2016). Fish Pathology in Perspective. Boga Press.

Abubakar, M.I., Adeshina, I., Ayeloja, A.A., & Abdulraheem, I. (2019). Induction of Normocytic-Normochromic Anaemia in C. gariepinus (Burchell, 1822) Exposed to Acute Toxicity of 1,1-Dimethyl 4,4-Bipiridillium (Paraquat) Under Laboratory Conditions. FULafia Journal of Science and Technology, 5(2), 46-52.

Ada, F.B., Ekpenyong, E., & Ayotunde, E.O. (2012). Haematological, biological and behavioural changes in Oreochromis niloticus (Linne 1757) juveniles exposed to paraquat herbicide. Journal of Environmental Chemistry and Ecotoxicology, 4(3), 64-74.

Adhikari, S., Sarkar, B., Chatterjee, A., Mahapatra, C.T., & Ayyappan, S. (2004) Effects of Cypermethrin and Carbofuran on Certain Hematological Parameters and Prediction of Their Recovery in a Freshwater Teleost, Labeorohita (Hamilton). Ecotoxicology and Environmental Safety, 58(2), 220-226.

Agha-Mohammadi, M., Farrokhi, F., & Tokmechi, A. (2012) Study the toxic effects of various concentrations of the herbicide Paraquat on Liver Tissue of Farmed Rainbow Trout.

Aghoghovwia, O.A., & Izah, S.C. (2018). Acute toxicity of Paraquat dichloride based herbicide against Heterobranchus bidorsalis fingerlings. EC Agriculture, 4(2), 128-132.

Auta, J., Balogun, J.K., & Ipinlolu, J.K. (2002). Short-term effects of dimethoate on behaviour of juveniles of Oreochromis niloticus (Trewava) C. gariepinus (Tengel). Journal of Tropics Biosciences, 2(1), 55-59

Babatunde, M.M., Balogun, J.K., & Oladimeji, A.A., (2014): Some haematological responses of O.niloticus to acute and sublethal concentration of paraquat. International Journal of Fisheries and Aquatic Studies, 1(5), 206-215.

Bahmani, M., Kazemi, R., & Donskaya, P. (2001) A comparative study of some hematological features in young reared sturgeon. Fish Physiology and Biochemistry, 24, 135-140.

Bahmani, M., Kazemi, R., Yousefi, A., Hallajian, A., & Mojazi-Amiri, B. (2008). The final Project Report: Studying the Possibility of Artificial Reproduction of Farmed Stellate Sturgeon (Broodstock, Artificial Reproduction and Production of Fingerlings from Farmed Sturgeon Broodstocks). Iranian Fisheries Research Organization Press.

Bagheri, F. (2007). Study of Pesticide Residues (Diazinon, Azinphosmethyl) in the Rivers of Golestan Province (Gorganroud and Gharehsou) [M. Sc Thesis, Tehran University of Medical Science].

Banaee, M., Mirvagefei, R., Rafei, G., & Majazi-Amiri, B. (2008) Effect of Sub-Lethal Diazinon Concentrations on Blood Plasma Biochemistry. International Journal of Environmental Research, 2(2), 189-198.

Banaee, M., Davoodi, M.H., & Zoheiri, F. (2013). Histopathological changes induced by paraquat on some tissues of gourami fish (Trichogaster trichopterus). Open Veterinary Journal, 3(1), 36-42.

Dogan, D., & Can, C. (2011). Hematological, biochemical, and behavioral responsesof Oncorhynchus mykiss to dimethoate. Fish Physiology and Biochemistry, 37, 951-958.

El-Sayed, Y.S., Saad, T.T., & El-Bahr, S.M.A. (2007). Acute intoxication of deltamethrin in mono-sex Nile tilapia, Oreochromis niloticus with special reference to the clinical, biochemical and haematological effects. Environmental Toxicology and Pharmacology, 24(3), 212-217.

Ezeri, G.N.O. (2001), Haematological responses of C. gariepinus to bacterial infection and prophylactic treatment with antibiotic. Journal of Aquacultural Sciences, 16(1), 22-24.

Gao, R., Choi, N., Chang, S.I., Kang, S.H., Song, J.M., Cho, S.I., Lim, D.W., & Choo, J. (2010). Highly sensitive trace analysis of paraquat using a surface-enhanced Raman scattering microdroplet sensor. Analytica Chimica Acta, 681(1-2), 87-91.

Goldberger, J., Wheeler, G.A., Lillie, R.D., & Rogers, L.M. (2001), A Study of the Pellagra-preventive action of Canned Salmon. Public Health Reproduction, xliv, 2769.>view>cdc>cdc_68372_DS1

Huang, C., Zhang, X., Jiang, Y., Li, G., Wang, H., Tang, X., & Wang Q. (2013). Paraquat induced convulsion and death: a report of five cases. Toxicology and Industrial Health, 29(8), 722-727.

John, P.J. (2007). Alterations of certain blood parameters of freshwater teleost Mystus vittatus after chronic exposure to Metasystox and Sevin. Fish Physiology and Biochemistry, 33, 15-20.

Joshi, P. K., Harish, D., & Bose, M. (2002). Effect of lindane and malathion exposure to certain blood parameters in a freshwater teleost fish Clarias batrachus. Pollution Resources, 21(1), 55-57.

Katagi T. (2010) Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms. In D. Whitacre (Ed.), Reviews of Environmental Contamination and Toxicology (Continuation of Residue Reviews) (vol 204, pp. 1-132). Springer.

Kori-Siakpere, O., Adamu, K.M., & Madukelum, I.T. (2007). Acute heamatological effects of sublethal levels of paraquat on the African catfish, C. gariepinus. Research journal of Environmental Sciences, 1(6), 331-335.

Kumar, N., Antony, P., Pal, A.K., Remya, S., Aklakur, M., Rana, R.S., Gupta, S., Raman, R.P., & Jadhao, S.B. (2011). Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of Endosulfan. Pesticide Biochemistry and Physiology, 99(1), 45-52.

Kumar, S., Sahu, N.P., Pal, A.K., Choudhury, D., Yengkokpam, S., & Mukherjee, S.C. (2015) Effect of Dietary Carbohydrate on Haematology, Respiratory Burst Activity and Histological Changes in L. rohita Juveniles. Fish and Shellfish Immunology, 19(4), 331-344.

Li, Z.H., Velisek, J., Zlabek, V., Grabic, R., Machova J., Kolarova, J., & Randak, T. (2011). Hepatic antioxidant status and hematological parameters in rainbow trout, Oncorhynchus mykiss, after chronic exposure to carbamazepine. Chemico-Biological Interactions, 183(1), 98–104.

Mallum, S.S., & Sogbesan, O.A (2015). Haematological assessment of Oreochromis niloticus (Linn, 1787) exposed to acute toxicity of herbicide propanil. International journal of Research and Life Science, 2(10), 49-56

Nussey, G., van Vuren, H.J., & du Preez, H.H. (2015). Effect of Copper on the Differential White Blood Cell Counts of Mozambique tilapia (Oreochromis mossambicus). Comparative Biochemistry and Physiology part C: Pharmacology, Toxicology and Endocrinology, 111(3), 381-388.

Omoregie, E., & Oyebanji, O. (2002). Oxytetracycline-induced blood disorder in Nile tilapia, Oreochromis niloticus (Trewavas). Journal of the World Aquaculture Society, 33(3), 377-382.

O’Neal, C.C., & Weirich, C.R. (2001). Effects of low levels salinity on production and haematological parameters of channel catfish, Ictalurus punctatus reared in multicropponds, In: Book of abstract. Aquaculture 2001. International Triennal Conference of world Aquaculture Society. Jan. 21-25. Florida, 484pp.

Osman, A.G.M., & Kloas, W. (2010). Water Quality and Heavy Metal Monitoring in Water, Sediments, and Tissues of the African Catfish C. gariepinus (Burchell, 1822) from the River Nile, Egypt. Journal of Environmental Protection, 1, 389-400.

Patnaik, I., & Patra, A.K. (2006). Haematopoietic alterations induced by carbaryl in Clarias batrachus (Linn). Journal of Applied Sciences and Environmental Management, 10(3), 5-7.

Pereira, L., Fernandes, M.N., & Martinez, C.B. (2013). Hematological and biochemical alterations in the fish Prochilodus lineatus caused by the herbicide clomazone. Environment Toxicology and Pharmacology, 36(1), 1-8.

Prusty, A.K., Kohli, M.P.S. Sahu, N.P., & Pal, A.K. (2011). Effect of short term exposure to fenvalerate on biochemical and haematological responses in Labeo rohita (Hamilton) fingerlings. Pesticide Biochemistry and Physiology,100(2), 124-129.

Saravanan, M., Kumar, K.P., & Ramesh, M. (2011). Haematological and biochemical responses of freshwater teleost fish Cyprinus carpo (Actinopterygii Cypriniformes) during aute and chronic sublethal exposure to Lindane. Pesticide Biochemistry and physiology, 100(3), 206-211

Saravanan, M., Usha-Devi, K., Malarvizhi, A., & Ramesh, M. (2012). Effects of Ibuprofen on hematological, biochemical and enzymological parameters of blood in an Indian major carp, Cirrhinus mrigala. Environmental Toxicology and Pharmacology, 34(1), 14-22.

Thakur, P.B., & Bais, V.S. (2000). Toxic effect of aldrin and fenvalerate on certain haematological parameters of a fresh water teleost, Heteropneustes fossils (Bl.). Journal of Environmental Biology, 21(2), 161-163.

Tsai, W.T. (2013). A review on environmental exposure and health risks of herbicide paraquat. Toxicological & Enviromental Chemistry, 95(2), 197-206.

Tortorelli, M.C, Hernández, D.A., Vázquez G., & Salibián, A. (1990). Effects of paraquat on mortality and cardiorespiratory function of catfish fry Plecostomus commersoni. Archives of Environmental Contamination and Toxicology, 19, 523-529.



How to Cite

Idi-Ogede Abubakar, M., & Ibrahim, A. (2021). Macrocytic-normochromic anaemia in the African catfish Clarias gariepinus (Siluriform: Clariidae) exposed to Paraquat under laboratory conditions. UNED Research Journal, 14(1), e3789.