Bacterias cultivables resistentes a oxitetraciclina durante el proceso de vermicompostaje de excretas bovinas

Bacterias cultivables resistentes a oxitetraciclina durante el proceso de vermicompostaje de excretas bovinas

Autores/as

DOI:

https://doi.org/10.22458/urj.v15i1.4586

Palabras clave:

antimicrobianos, tetraciclina, estiércol, bovino, lombricompost, té de lombricompost

Resumen

Introducción: A nivel mundial se han detectado bacterias con genes de resistencia al antibiótico oxitetraciclina en estiércol de vacas lecheras, sin embargo, es una práctica común utilizar este estiércol como fertilizante. El vermicompostaje puede reducir el problema, pero este tema no ha sido evaluado en Costa Rica. Objetivo: Analizar la presencia de bacterias resistentes a la oxitetraciclina durante el vermicompostaje en una finca lechera costarricense. Métodos: A partir de estiércol fresco, precompostado, lombricomposta, y tés de composta con y sin melaza y tés de composta con y sin melaza, inoculamos caldos suplementados con oxitetraciclina. Extrajimos el ADN de estos cultivos líquidos y secuenciamos en masa el amplicón ribosómico 16S. Resultados: Clasificamos 105 292 secuencias en 58 variantes de secuencia de amplicón de caldos suplementados con oxitetraciclina, la mayoría identificadas como Proteobacteria, Bacteroidetes y Firmicutes. El estiércol fresco tuvo más bacterias resistentes (32), seguido de los “tés” sin y con melaza. El precompostaje y el vermicompostaje disminuyeron la cantidad y variedad de bacterias resistentes. Sin embargo, la preparación de los tés provocó la multiplicación de géneros bacterianos reconocidos por su capacidad de acumular determinantes de resistencia. Conclusión: El precompostaje y el vermicompostaje disminuyeron el número y tipo de bacterias resistentes.

 

Citas

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Publicado

2023-05-31

Cómo citar

Uribe-Lorío, L., WingChing-Jones, R., Vidaurre-Barahona, D., Uribe, L., & Aráuz, L. F. (2023). Bacterias cultivables resistentes a oxitetraciclina durante el proceso de vermicompostaje de excretas bovinas. UNED Research Journal, 15(1), e4586. https://doi.org/10.22458/urj.v15i1.4586

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