Morphogenetic responses of cuttings and in vitro plants of Vanilla planifolia (Orchidaceae) during initial development in greenhouse and agroforestry systems

Morphogenetic responses of cuttings and in vitro plants of Vanilla planifolia (Orchidaceae) during initial development in greenhouse and agroforestry systems

Authors

  • José Bernal Azofeifa Bolaños Universidad Nacional de Costa Rica
  • German Rivera Coto Universidad Nacional de Costa Rica
  • Amelia Paniagua Vásquez Universidad Nacional de Costa Rica
  • Roberto Cordero Solórzano Universidad Nacional de Costa Rica

DOI:

https://doi.org/10.22458/urj.v10i2.1995

Keywords:

vanilla, wild type relatives, domestication, Costa Rica

Abstract

There is little knowledge in Costa Rica about morphogenetic responses of Vanilla planifolia. Here we compare cutting and micro-cutting for morphogenetic response in nursery and agroforestry. Each plant type was grown in both conditions and the combination with factor levels resulted in four treatments. The independent growth responses of the cuttings were significantly higher for the length, total weight and emergent shoot, total and average length and weight of the roots compared to that in micro-cuttings. For this factor, a significant increase of nodes was achieved when micro-cuttings were used. The length, total weight and emergent shoot, weight and number of roots of the explants grown in the field were significantly greater compared to that in nursery. Total and average length of the roots did not significantly differ between independent treatments of condition factor. Significant interaction between factors were obtained for all variables except for the total weight and the emergent shoot. After a period of nursery acclimatization, the agroforestry vitroplants were significantly higher for the length of the shoot, number of nodes and number of roots in relation to the cuttings grown in both conditions, as well as the vitroplants grown in nursery. Longer and heavier roots were obtained when cuttings were grown at the nursery, suggesting a more intense growth in the search for water and nutrients.

Author Biographies

José Bernal Azofeifa Bolaños, Universidad Nacional de Costa Rica

Departamento de Agroforestería, Investigador

German Rivera Coto, Universidad Nacional de Costa Rica

Laboratorio de Fitopatología, Catedrático

Amelia Paniagua Vásquez, Universidad Nacional de Costa Rica

Departamento de Agroforestería, Extensionista

Roberto Cordero Solórzano, Universidad Nacional de Costa Rica

Escuela de Ciencias Biológicas, Investigador

References

Adugna, M., Belew, D., & Tilahun, D. (2015). Influence of rooting media and number of nodes per stem cutting on nursery performance of vanilla (Vanilla planifolia Andr. syn. Vanilla fragrans). Journal of Horticulture and Forestry, 7(3), 48–56. doi:10.5897/JHF2014.0376

Arango, D. A., & Moreno, F. (2011). Desarrollo inicial de la vainilla (Vanilla planifolia Andrews, Orchidaceae) bajo diferentes usos de la tierra y condiciones climáticas en Colombia. In I. Lombardi, W. Nalvarte, W. Ojeda, E. Hilario, C. Pajuelo, W. Echeverría, … J. Saravia (Eds.), Congreso Forestal Latinoamericano pp. 1–17. Lima, Perú: Cámara Nacional Forestal. Recuperado de: http://www.cnf.org.pe/secretaria_conflat/memorias/INDICE.pdf

Azofeifa-Bolaños, J. B., Gigant, L. R., Nicolás-García, M., Pignal, M., Tavares-González, F. B., Hágsater, E., … Grisoni, M. (2017). A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae). European Journal of Taxonomy, 284, 1–26. doi:10.5852/ejt.2017.284

Azofeifa-Bolaños, J. B., Paniagua-Vásquez, A., & García-García, J. A. (2014). Importancia y desafíos de la conservación de Vanilla spp. (Orchidaceae) en Costa Rica. Agronomía Mesoamericana, 25(1), 189–202. doi:10.15517/am.v25i1.14220

Baltazar-Nieto, P. (2010). Caracteres morfológicos de vainilla utilizados por el agricultor en la selección del material reproductivo en cuatro municipios del Totonacapan, México. Colegio de Postgraduados,México.

Belanger, F.C., & Havkin-Frenkel, D. (2011). Molecular analysis of a Vanilla hybrid cultivated in Costa Rica. In: D. Havkin-Frenkel, & F.C. Belanger. (Eds), Handbook of Vanilla Science and Technology pp. 256– 263. Wiley-Blackwell, Chichester, United Kingdom. doi:0.1002/9781444329353

Bello-Bello, J. J., Martínez-Estrada, E., Caamal-Velázquez, J. H., & Morales-Ramos, V. (2016). Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews). African Journal of Biotechnology, 15(8), 272–277. doi:10.5897/AJB2015.14662

Chen, H., Mendelssohn, I. A., Lorenzen, B., Brix, H., & Miao, S. (2005). Growth and nutrient responses of Eloecharis cellulosa (Cyperaceae) to phosphate level and redox intensity. American Journal of Botany, 92(9), 1457–1466. doi:10.3732/ajb.92.9.1457

FDA (Food and Drug Administration). (2017). Food Dressings and Flavorings. Recuperado de: https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?CFRPart=169&showFR=1&subpartNode=21:2.0.1.1.44.1

Giridhar, P., & Ravishankar, G. A. (2004). Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk. Indian Journal of Biotechnology, 3, 113–118.

Gómez, N. (2012). Respuestas de Vanilla planifolia Jacks. ante variaciones microambientales bajo arreglos agroforestales en un bosque seco tropical. Universidad Nacional de Colombia. Recuperado de: http://www.bdigital.unal.edu.co/8273/1/43455134.pdf

Hailemichael, G., Tilahun, D., Kifelw, H., & Mitiku, H. (2012). The effect of different node number cuttings on nursery performance of Vanilla (Vanilla planifolia syn. Vanilla fragrans) in south western Ethiopia. International Research Journal of Agricultural Science and Soil Science, 2(9), 408–412.

IMN. (2011). Promedios mensuales de datos climáticos Estación Automática Finca Diamantes, Guápiles, Limón. Gestión de Información y Comercialización. San José, Costa Rica.

Janarthanam, B., & Seshadri, S. (2008). Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr. In Vitro Cellular & Developmental Biology - Plant, 44(2), 84–89. doi:10.1007/s11627-008-9123-4

Lee-Espinosa, H. E., Murguía-González, J., García-Rosas, B., Córdova-Contreras, A. L., Laguna-Cerda, A., Mijangos-Cortés, J. O., … & Santana-Buzzy, N. (2008). In vitro clonal propagation of vanilla (Vanilla planifolia “Andrews”). HortScience, 43(2), 454–458.

Monnin, M. E., & Perret, J. (2013). Absorción de los nutrientes y crecimiento de la vainilla (Vanilla planifolia). Tierra Tropical, 9(1), 29–37. Recuperado de: http://tierratropical.org/es/editions/issue-9-1-2013/absorption-of-nutrients-and-growth-of-vanilla-vanilla-planifolia/

Ordóñez, N. F., Otero, J. T., & Díez, M. C. (2012). Hongos endófitos de orquídeas y su efecto sobre el crecimiento en Vanilla planifolia Andrews. Acta Agronómica, 61(3), 282–290.

Osorio, A. (2012). Efecto de materiales orgánicos, fertilizantes e inóculos microbiales sobre el crecimiento y nutrición de plántulas de vainilla (Vanilla planifolia Jacks). Universidad Nacional de Colombia.

Osorio, A. I., Osorio, N. W., Díez, M. C., & Moreno, F. (2014). Nutrient status and vegetative growth of Vanilla planifolia Jacks plants as affected by fertilization and organic substrate composition. Acta Agronómica, 63(4), 326–334. doi:10.15446/acag.v63n4.40754

Osorio, A. I., Osorio, N. W., Diez, M. C., & Moreno, F. H. (2012). Effects of organic substrate composition, fertilizer dose, and microbial inoculation on vanilla plant nutrient uptake and growth. Acta Horticulturae, 964, 135–142. doi:10.17660/ActaHortic.2012.964.17

Quesada, R. (2007). Los Bosques de Costa Rica. In Instituto Tecnológico de Costa Rica (Ed.), lX Congreso Nacional de Ciencias Exploraciones: Exploraciones fuera y dentro del aula pp. 1–16. Cartago, CR: Instituto Tecnológico de Costa Rica.

Shao, H. B., Chu, L. Y., Jaleel, C. A., & Zhao, C. X. (2008). Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus - Biologies, 331(3), 215–225. doi:10.1016/j.crvi.2008.01.002

Shen, J., Li, C., Mi, G., Li, L., Yuan, L., Jiang, R., & Zhang, F. (2013). Maximizing root/rhizosphere efficiency to improve crop productivity and nutrient use efficiency in intensive agriculture of China. Journal of Experimental Botany, 64(5), 1181–1192. doi:10.1093/jxb/ers342

Soto-Arenas, M. A. (1999). Filogeografía y recursos genéticos de las vainillas de México. México, D. F.

Soto-Arenas, M. A., & Solano-Gómez, A. R. (2007). Ficha técnica de Vanilla planifolia. In M. A. Soto-Arenas (Ed.), Información actualizada sobre las especies de orquídeas del PROY-NOM-059-ECOL- 2000. (pp. 1–18). México, D. F.: Instituto Chinoin A.C., Herbario de la Asociación Mexicana de Orquideología A.C. Bases de datos SNIB-CONABIO. Proyecto No. W029. Recuperado de: http://www.conabio.gob.mx/conocimiento/ise/fichasnom/Vanillaplanifolia00.pdf

Tan, B. C., Chin, C. F., & Alderson, P. (2011). An Improved Plant Regeneration of Vanilla planifolia Andrews. Plant Tissue Culture and Biotechnology, 21(1), 27–33.

Umesha, K., Murthy, G., & Smitha, G. R. (2011). Environmental conditions and type of cuttings on rooting and growth of vanilla (Vanilla planifolia Andrews). Journal of Tropical Agriculture, 49(1–2), 121–123. Recuperado de: http://jtropag.kau.in/index.php/ojs2/article/view/251/251

Published

2018-09-21

How to Cite

Azofeifa Bolaños, J. B., Rivera Coto, G., Paniagua Vásquez, A., & Cordero Solórzano, R. (2018). Morphogenetic responses of cuttings and in vitro plants of Vanilla planifolia (Orchidaceae) during initial development in greenhouse and agroforestry systems. UNED Research Journal, 10(2), 368–378. https://doi.org/10.22458/urj.v10i2.1995

Issue

Section

Articles
Loading...