Use of bacteria (Azospirrillum spp.) as biofertilizer for African star grass (Cynodon nlemfuensis)
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Keywords

Fertilization
biofertilizer
dry matter
yield
dairy cattle
tropical grassland.

How to Cite

WingChing-Jones, R., Uribe Lorío, L., & Castro Barquero, L. (2016). Use of bacteria (Azospirrillum spp.) as biofertilizer for African star grass (Cynodon nlemfuensis). UNED Research Journal, 8(2), 259-265. https://doi.org/10.22458/urj.v8i2.1570

Abstract

African star grass is important in some tropical regions and can benefit from the use of biofertilizers like Azospirillum. We tested three strains of Azospirillum as biofertilizer in Turrialba, Costa Rica, from 2011 through 2013. A single dose of 5 liters per hectare was used for the three strains (108 CFC.ml-1) and a three-way mixture of the strains; and compared with a dose of 10 tons of vermicompost and chemical fertilization (78 kg N per hectare). A second experiment evaluated Azospirillum for its potential to substitute chemical fertilizer in proportions of 0, 25, 50 and 75%. A dose of 5 L/ha (108 CFU.ml-1) was used for all treatments and compared to the dose of 100% of chemical fertilizer. Two of the strains and the three-way yielded similar forage biomass (3.65 to 3.93 t/ha) as the chemical fertilizer (4.12 t/ha). Fertilization with vermicompost was lower (3.56 t/ha). A decline in rainfall from 2012 to 2013 reduced the grass biomass 25% when chemical fertilization was applied (4.07 vs 3.08 t/ha). In the second experiment, no significant differences were found in biomass yields for all treatments with Azospirillum or chemical fertilizer (2.97 vs 3.08 t/ha). Azospirillum has potential to maintain the productivity of African star grass under a grazing system, but further research is required to define the number of applications per year to maintain a constant effect.
https://doi.org/10.22458/urj.v8i2.1570
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References

Aguilar-Piedras, J., Xiqui-Vásquez, M., Garcia-Garcia, S. & Baca, B. (2008). Producción del ácido indol-3-acético en Azospirillum. Revista Latinoamericana de Microbiología, 50, 29-37.

AOAC (Association of Official Analytical Chemist). (1998). Official methods of analysis of AOAC International. 16th ed, 4th rev. Gaithersburg, MD: AOAC International, USA.

Baldani, J.I., Krieg, N.R., Baldani, V.L.D., Hartman, A. & Dobereiner, J. (2005). Genus II Azospirillum in Bergey’s Manual of Systematic Bacteriology. Second edition, vol 2. The Proteobacteria. Part C. The Alpha-Beta and Epsilom proteobacteria, Benner, D.J., Kreig, N.R. and Staney, J.T. Eds. New York: Bergey´s Manual Trust, pp. 7-26.

Barassi, C.A., Sueldo, R.J., Creus, C.M., Carrozzi, L.E., Casanovas, E.M & Pereyra, M.A. (2007). Azospirillum spp., a dynamic soil bacterium favorable to vegetable crop production dynamic soil. Dynamic Plant, 1, 68-82.

Bashan, Y. & de Baschan, L.E. (2010). How the plant grow promoting bacterium Azospirillum promotes plant growth.-A critical assessment. Advances in Agronomy, 8, 77-136.

Bashan, Y, de Basshan, L.E., Prabhu, S. R. & Hernández, J.P. (2014). Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant Soil, 378,1–33

Bhattacharya, P.N. & Jha, D. K. (2012). Plant growth-promoting rhizobacteria (PGPR): emergence in agricultura Review. World J Microbiol Biotechnol, 28,1327–1350.

García-Olivares, J., Moreno-Medina, A V., Rodríguez-Luna, C., Mendoza-Herrera, A. & Mayek-Pérez, N. (2006). Azospirillum brasilense biofertilization in sorghum at northern México. Agricultura Técnica en México, 32 (2). 135-141.

Giller, K.E. & Wilson, K.J. (1991). Nitrogen fixation in tropical cropping systems. CAB International. United Kingdom. pp. 19-20.

Hungría, M., Campo, R. J., Souza, E. M. & Pedrosa, F.O. (2010). Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil, 331, 413-425.

Instituto Nacional de Estadística y Censos, INEC. (2015). VI Censo nacional agropecuario: Resultados generales/Instituto Nacional de Estadísticas y Censo. -1ed- San José, Costa Rica. 146 p.

Itzigsohn, R., Burdman, S., Okon, Y., Zaady, E., Yonatan, R. & Perevolotsky, A. (2000). Plant-growth promotion in natural pastures by inoculation with Azospirillum brasilense under suboptimal growth conditions. Arid Soil Res. Rehab, 13,151-158.

Kapulnik, Y., Okon, Y., Kigel, J., Nur, I. & Henis, Y. (1981). Effects of temperature, nitrogen fertilization, and plant age on nitrogen fixation by Setaria italica inoculated with Azospirillum brasilense (strain cd). Plant Physiol, 68, 340-343.

Kaymak, H. C. (2011). Potential of PGPR in agricultural innovations. pp. 45-79. In: Plant growth and health promoting bacteria. Springer Berlin Heidelberg.

Kennedy, I.R., Choudhury, A.T.M.A. & Kecskes, M.L. (2004). Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biological and Biochemistry, 36,1229-1244.

Martínez-Morales, L.J., Soto-Urzúa, L., Baca, B. E. & Sánchez, J. A. (2003). Indole-3-butyric acid (IBA) production in culture medium by wild strain Azospirillum brasilense. FEMS Microbiol. Lett, 228(2),167-173.

Mehnaz, S. (2015). Azospirillum. A biofertilizer for every crop. pp:297-314. Arora, N.K. (ed) Plant microbes symbiosis: Applied facets. DOI: 10.1007/978-81-322-2068-8.15. Springer India.

Okon, Y. & Labandera-González, C. A. (1994). Agronomic applications of Azospirillum: an evaluation of 20 years worldwide field inoculation. Soil Biol. Biochem, 26, 1591-1601.

Podile, A. R. & Kishore, G. K. (2006). Plant growth-promoting rhizobacteria. In: Plant-associated bacteria. Springer Netherlands. p. 195-230.

Ramírez, C., Soto, Z., Castro, L., Arauz, L.F., Uribe-Lorío L. & Uribe, L. (2015). Efecto de cuatro rizobacterias promotoras de crecimiento sobre la pudrición basal causada por Phytophthora capsici en plantas de chile dulce (Capsicum annuum). Agronomía Costarricense, 39(3), 87-100.

SAS 2003. SAS 9.1.3. for Windows. Service Pack 4. Win_Pro plataforma. Copyright® 2002-2003 Cary NC.

Salazar, S. (2007). Disponibilidad de biomasa y valor nutricional del pasto Estrella africana (Cynodon nlemfuensis) en el distrito de Quesada, Cantón de San Carlos. Tesis de licenciatura. Universidad de Costa Rica, Costa Rica. 96 p.

Tsavkelova, E. A., Klimova, S. Y., Cherdyntseva, T. A. & Netrusov, A. I. (2006). Microbial Producers of Plant Growth Stimulators and Their Practical Use: A Review. Applied Biochemistry and Microbiology, 42 (2),117–126.

Umali-Garcia, M., Hubbell, D.H., Gaskins, M.H.& Dazzo, F.B. (1984). Adsorption and mode of entry of Azospirillum brasilense to grass roots, pp 49-62. In: P. Vose y A. Ruschel (eds.). Associative N2-Fixation. Volume I. CRC Press. Florida.

Veresolou, S.D. & Menexes, G. (2010). Impact of inoculation with Azospirillum spp. on growth properties and seed yield of wheat: a meta-analysis of studies in the ISI Web of Science from 1981 to 2008. Plant Soil, 337, 469-480.

Villalobos, L., Arce, J. & WingChing, R. (2013). Producción de biomasa y costos de producción de pastos estrella africana (Cynodon nlemfuensis), kikuyo (Kikuyuocloa clandestina) y ryegrass perenne (Lolium perenne) en lecherías de Costa Rica. Agronomía Costarricense, 37(2), 91-103.

Vivienne, N., Matiru, F.D. & Dakora, S. (2004). Potential use of rhizobial bacteria as promoters of plant growth for increased yield in landraces of African cereal crops. Afr. J. Biotechnol, 3(1),1-7.

WingChing-Jones, R., Villalobos, L., Arce, J. & Rojas, A. (2013). Informe parcial proyecto de investigación 739-B2-082. Condiciones ambientales y producción de forraje del pasto Estrella Africana. Vicerrectoría de Investigación, Universidad de Costa Rica, Costa Rica. 6 p

Xie, C.H. & Yokota, A. (2005). Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa. International Journal of Systematic and Evolutionary Microbiology, 55,1435-1438.

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