Sensibilidad a los antibióticos en bacterias Gram negativas de bulbos de cebolla en Tierra Blanca de Cartago
DOI:
https://doi.org/10.22458/rc.v20i2.2395Palabras clave:
Perfil de sensibilidad a antibióticos, gentamicina, tetraciclina, cebolla, bacteriaResumen
El objetivo de este estudio fue identificar géneros bacterianos en bulbos de cebolla sanos y bulbos que presentaban síntomas, de la zona de Tierra Blanca de Cartago y determinar la susceptibilidad a los antibióticos gentamicina y tetraciclina, dos de los antibióticos más usados en la agricultura en Costa Rica. El cultivo de la cebolla se practica en diferentes regiones del país, principalmente en la zona de Cartago. Este cultivo puede ser atacado por una gran variedad de plagas y enfermedades que pueden disminuir el crecimiento y la producción, causar daños y desfigurar los bulbos impidiendo su comercialización. Las bacterias que atacan los cultivos causan grandes pérdidas económicas, por eso se han buscado diversos métodos para controlarlas, entre ellos, el uso de antibióticos como tetraciclina y gentamicina. La selección y diseminación en la naturaleza de cepas resistentes a los antibióticos es una práctica que se debe controlar, con el fin de mantener un balance ecológico que favorezca el predominio de bacterias susceptibles y asegurar el tratamiento efectivo de las enfermedades infecciosas humanas.
Citas
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Bishop A.L., Davis R.M. (1990). Internal decay of onions caused by Enterobacter cloacae. Plant Dis. 74:692-694.
Brewster J.L. (2008). Onions and other vegetable alliums. Crop production sciences in horticulture. 2nd edition. N.15 CAB International, USA. 171.
Chatterjee A.K., Starr M.P. (1972). Transfer among Erwinia spp. and other enterobacteria of antibiotic resistance carried on R Factors. J. Bacteriol. 112:576-584.
Cother E.J., Dowling V. (1986). Bacteria associated with internal breakdown of onion bulbs and their possible role in disease expression. Plant Pathol. 35:329-336.
Edens D.G., Gitaitis R.D., Sanders F.H., Nischwitz C. (2006). First report of Pantoea agglomerans causing a leaf blight and bulb rot of onions in Georgia. Plant Dis 90:1551.
FAO. 2015. http://faostat.fao.org/site/567/DesktopDefault. aspx?PageID=567#ancor, consultado el 28 de agosto de 2015
Flaherty J.P., García-Houchins S., Chudy R., Arnow P.M. (1993). An outbrake of Gram-negative bacteremia traced to contaminated O-Rings in reprocessed dialyzers. Ann. Intern. Med. 119:1072-1078.
Frost L.S., Leplae R., Summers A.O., Toussanint A. (2005) Mobile genetic elements: The agents of open source evolution. Nat Rev Microbiol. 3: 722-732.
Galt R.E. (2009). It just goes to kill Ticos: national market regulation and the political ecology of farmers’ pesticide use in Costa Rica. J. Pol. Ecol.16:1-33.
Galland J., Hyatt D., Crupper S., Acheson D. (2001). Prevalence, antibiotic susceptibility and diversity of Escherichia coli O157:H7 isolates from a longitudinal study of beefcattle feedlots. Appl Environ Microbiol.
67:1619-1627.
Gallardo F., Ruiz J., Marco F., Towner K.J., Vila J. (1999) Increase in incidence of resistance to ampicillin, chloramphenicol and trimethoprim in clinical isolates of Samonella serotype typhimurium with investigation of molecular epidemiology and mechanisms of resistance. J Med Microbiol 48:367-74.
Goszczynska T. (2007). Emerging diseases of maize and onion caused by bacteria belonging to the Genus Pantoea. The Faculty of Natural and Agricultural Sciences University of Pretoria. Pretoria, SAF. 5-12p.
Granados M. (2013). Problemas fitosanitarios de la cebolla en Costa Rica. Primera edición. Facultad de Ciencias Agroalimentarias. Universidad de Costa Rica, San José, Costa Rica. 1-8.
Grunda N. (2005). Impact of environmental factors on productn quality of greenhouse vegetables for fresh consumption. Crit. Rev. Plant Sci. 24:227-247
Hanning I., Nutt J.D., Ricke S. (2009). Salmonellosis Outbreaks in the United States Due to Fresh Produce: Sources and Potential Intervention Measures. Foodborne pathogens and disease. 6: 635-648.
Izard D., Ferragut C., Gavini F., Kersters K., De Ley J., Leclerc H. (1981). Klebsiella terrigena, a new species from soil and water. Int J Syst Bacteriol. 31:116-127.
Joaquín A., Khna S., Russel N., Al Fayez N. (1991). Neonatal meningitis and bilateral cerebellar abscesses due to Citrobacter freundii. Pediatr. Neurosurg. 17:23-24.
Levy S.B. (1998). The challenge by antibiotic resistance. Sci Am. 278:46-53.
Levy S.B. (2004). Antibacterial resistance worldwide: Causes, challenges and responses. Nature Med. 10
(Suppl): 122-129.
MAG (Ministerio de Agricultura y Ganadería). (2007). Caracterización de la agro-cadena regional de cebolla. San José, CRC. 1-31.
Mahon C., Lehman D., Manuselis G. (2007). Textbook of diagnostic microbiology. Elsevier. St Louis, MO.
USA. 433-440.
Maloy O.C. 2005. Plant disease management. The Plant Health Instructor: file:///E:/TFG%20Gentamicina/ Plant%20Disease%20Management.htm. DOI: 10.1094/PHI-I-2005-0202-01.
Mc Manus P.S., Stockwell V.O., Sundin G.W., Jones A.L. (2002). Antibiotic use in plant agriculture. Annu. Rev.
Phytopathol. 40:443-465.
Miko A., Pries K., Schoetes A., Helmuth R. (2005). Molecular mechanisms of resistance in multidrug-resistant
ser. of Salmonella enterica isolated from foods in Germany. J. Antimicrobial Chem. 56:1025-1033.
Miskimin D.K., Berkowits K., Solberg M., Riha W.E., Frankie W.C., Buchanan R.L., O´leary V. (1976). Relationships between indicator organisms and specific pathogens in potenctially hazardous foods. J. Food.
Sci. 41:1001.
Murray P., Holmes B., Auken H. (2005). Citrobacter, Klebsiella, Enterobacter, Serratia, and other Enterobacteriaceae. In: P. Borriello et al., editors,Tropley and Wilson’s Microbiology and Microbial Infections. Volume 2. 10th ed. London: Hodder Arnold. p.1474-1507.
NCCLS. (National Committee of Clinical Laboratory Standard). (1997). Performance standard for antimicrobial disk susceptibility test, 6th ed. Approved Standard Documents M2. National Committee of Clinical Laboratory Standard, Wayne. PA, USA. 24-30p.
Pegues D.A., Ohl M.E., Miller S.I. (2002). Salmonella, including Salmonella typhi. In: Blaser MJ, Smith PD, Ravdin JI, Greenberg HB, Guerran RL (eds.). Infections of the gastrointestinal tract. 2nd ed. Philadelphia: Lippincott Williams & Wilkins. 669-697p.
PIMA (Programa Integral de Mercado Agropecuario). (2013). Tendencias del consumo de frutas, hortalizas y pescado en las familias de Costa Rica. Costa Rica. 35-45p.
Prescott L., Harley J., Klein D. (2004). Microbiología. Quinta edición. McGraw Hill Interamericana. Madrid,
España. 145-162p.
Pouch F., Ito K. (2001). Compendium of methods for the microbiological examination of foods. Fourth edition. American Public Health Association. WA, USA. 83-100p.
Rodríguez E., Coronado M., H F., GarciaJ. (2005). Bacteriología general: Principios y prácticas de laboratorio. Editorial Universidad de Costa Rica. Costa Rica. 134, 213, 217, 265p.
Sacsaquispe R., Velásquez J. (2002). Manual de procedimientos para la prueba de sensibilidad antimicrobiana por el método de difusión. Serie de normas técnicas N. 30. Instituto nacional de salud. Lima, PER.
Saldar A., Armstrong D. (2003). Antimicrobial activities against 84 Listeria monocytogenes isolates from patients with sistemic listeriosis at a comprensive cancer center (1955-1997). J Clinical Microbiol. 49:483-485.
Sayah R., Kaneene J., Johnson Y., Miller R. (2005). Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic and wild animal fecal samples, human sewage and surface water. Appl
Environ Microbiol. 71:1394-1404.
Schroeder B.K., Du Toit L.J., Schwartz H.F. (2009). First report of Enterobacter cloacae causing onion bulb rot in the Columbia Basin of Washington State. Plant Dis. 93: 323.
Schwartz H.F., Gent D. (2007). Onion soft rot. High Plains IPM Guide, a cooperative effort of the University of Wyoming, University of Nebraska, Colorado State University and Montana State University, USA.
Schwartz H.F., Mohan S.K. (2008). Compendium of onion and garlic diseases and pests, 2nd ed. American Phytopathological Society (APS Press), St. Paul, MN, USA.
Schwartz H.F., Otto K. (2000). First report of a leaf and bulb decay of onion by Pantoea ananatis in Colorado.
Plant Dis. 84:808.
SEPSA. Secretaría Ejecutiva de Planificación Sectorial Agropecuaria (SEPSA). 2014. Boletín Estadístico
Agropecuario Nº 25. Costa Rica. 17-40.
SFE. Servicio Fitosanitario del Estado (SFE). 2015. https://www.sfe.go.cr/insumosys/Principal.html. Consultado el 29 de agosto de 2015.
Silliker J.H., Gabis D.A. (1976). ICMSF methods studies. VII indicator tests as substitutes for direct testing
of dried foods and feeds for Salmonella. Can. J. Microbiol. 22:971.
Stockwell V.O., Duffy B. (2012). Use of antibiotics in plant agriculture. Rev. sci. tech.Off. int. Epiz., 31: 199-210
Tauxe R., Kruse H., Hedberg C. (1997). Microbial hazards and emerging issues associated with produce, a preliminary reportto the national advisory committee on microbiologic criteriafor foods. J Food Prot. 60:1400-1408.
Uzeh R.E., Alade F.A., Bankole M. (2009). The microbial quality of pre-packed mixed vegetable salad in some retail outlets in Lagos Nigeria. Africal Journal of Food Science. 3: 270-272.
Vavilov N.I. (1951). The origin, variation, immunity and breeding of cultivated plants. Chronicles of botany
3: 1.
Vidaver A.K. (2002). Uses of antimicrobials in plant agriculture. Clin. Infect. Dis. 34:107-110.
Walcott R.R., Gitaitis R.D., Castro A.C., Sanders F.H., Díaz-Pérez J.C. (2002). Natural infestation of onion seed by Pantoea ananatis, causal agent of center rot. Plant Dis 86:106-111.
Wang A. (2013). Enfermedades bacterianas de la cebolla. In: M. Granaddos (ed). Problemas fitosanitarios de
la cebolla en Costa Rica. Primera edición. Facultad de Ciencias Agroalimentarias,Universidad de Costa Rica, San José, Costa Rica.
Zaid A.M.A., Bonasera J.M., Beer S.V. (2011). First report of Enterobacter decay caused by Enterobacter cloacae in New York. Plant Dis. 95:1581.
Arauz F. (2011). Fitopatología, un enfoque agroecológico. Segunda edición. Editorial de la Universidad de Costa Rica. San José, Costa Rica. 434-437p.
Bishop A.L., Davis R.M. (1990). Internal decay of onions caused by Enterobacter cloacae. Plant Dis. 74:692-694.
Brewster J.L. (2008). Onions and other vegetable alliums. Crop production sciences in horticulture. 2nd edition. N.15 CAB International, USA. 171.
Chatterjee A.K., Starr M.P. (1972). Transfer among Erwinia spp. and other enterobacteria of antibiotic resistance carried on R Factors. J. Bacteriol. 112:576-584.
Cother E.J., Dowling V. (1986). Bacteria associated with internal breakdown of onion bulbs and their possible role in disease expression. Plant Pathol. 35:329-336.
Edens D.G., Gitaitis R.D., Sanders F.H., Nischwitz C. (2006). First report of Pantoea agglomerans causing a leaf blight and bulb rot of onions in Georgia. Plant Dis 90:1551.
FAO. 2015. http://faostat.fao.org/site/567/DesktopDefault. aspx?PageID=567#ancor, consultado el 28 de agosto de 2015
Flaherty J.P., García-Houchins S., Chudy R., Arnow P.M. (1993). An outbrake of Gram-negative bacteremia traced to contaminated O-Rings in reprocessed dialyzers. Ann. Intern. Med. 119:1072-1078.
Frost L.S., Leplae R., Summers A.O., Toussanint A. (2005) Mobile genetic elements: The agents of open source evolution. Nat Rev Microbiol. 3: 722-732.
Galt R.E. (2009). It just goes to kill Ticos: national market regulation and the political ecology of farmers’ pesticide use in Costa Rica. J. Pol. Ecol.16:1-33.
Galland J., Hyatt D., Crupper S., Acheson D. (2001). Prevalence, antibiotic susceptibility and diversity of Escherichia coli O157:H7 isolates from a longitudinal study of beefcattle feedlots. Appl Environ Microbiol.
67:1619-1627.
Gallardo F., Ruiz J., Marco F., Towner K.J., Vila J. (1999) Increase in incidence of resistance to ampicillin, chloramphenicol and trimethoprim in clinical isolates of Samonella serotype typhimurium with investigation of molecular epidemiology and mechanisms of resistance. J Med Microbiol 48:367-74.
Goszczynska T. (2007). Emerging diseases of maize and onion caused by bacteria belonging to the Genus Pantoea. The Faculty of Natural and Agricultural Sciences University of Pretoria. Pretoria, SAF. 5-12p.
Granados M. (2013). Problemas fitosanitarios de la cebolla en Costa Rica. Primera edición. Facultad de Ciencias Agroalimentarias. Universidad de Costa Rica, San José, Costa Rica. 1-8.
Grunda N. (2005). Impact of environmental factors on productn quality of greenhouse vegetables for fresh consumption. Crit. Rev. Plant Sci. 24:227-247
Hanning I., Nutt J.D., Ricke S. (2009). Salmonellosis Outbreaks in the United States Due to Fresh Produce: Sources and Potential Intervention Measures. Foodborne pathogens and disease. 6: 635-648.
Izard D., Ferragut C., Gavini F., Kersters K., De Ley J., Leclerc H. (1981). Klebsiella terrigena, a new species from soil and water. Int J Syst Bacteriol. 31:116-127.
Joaquín A., Khna S., Russel N., Al Fayez N. (1991). Neonatal meningitis and bilateral cerebellar abscesses due to Citrobacter freundii. Pediatr. Neurosurg. 17:23-24.
Levy S.B. (1998). The challenge by antibiotic resistance. Sci Am. 278:46-53.
Levy S.B. (2004). Antibacterial resistance worldwide: Causes, challenges and responses. Nature Med. 10
(Suppl): 122-129.
MAG (Ministerio de Agricultura y Ganadería). (2007). Caracterización de la agro-cadena regional de cebolla. San José, CRC. 1-31.
Mahon C., Lehman D., Manuselis G. (2007). Textbook of diagnostic microbiology. Elsevier. St Louis, MO.
USA. 433-440.
Maloy O.C. 2005. Plant disease management. The Plant Health Instructor: file:///E:/TFG%20Gentamicina/ Plant%20Disease%20Management.htm. DOI: 10.1094/PHI-I-2005-0202-01.
Mc Manus P.S., Stockwell V.O., Sundin G.W., Jones A.L. (2002). Antibiotic use in plant agriculture. Annu. Rev.
Phytopathol. 40:443-465.
Miko A., Pries K., Schoetes A., Helmuth R. (2005). Molecular mechanisms of resistance in multidrug-resistant
ser. of Salmonella enterica isolated from foods in Germany. J. Antimicrobial Chem. 56:1025-1033.
Miskimin D.K., Berkowits K., Solberg M., Riha W.E., Frankie W.C., Buchanan R.L., O´leary V. (1976). Relationships between indicator organisms and specific pathogens in potenctially hazardous foods. J. Food.
Sci. 41:1001.
Murray P., Holmes B., Auken H. (2005). Citrobacter, Klebsiella, Enterobacter, Serratia, and other Enterobacteriaceae. In: P. Borriello et al., editors,Tropley and Wilson’s Microbiology and Microbial Infections. Volume 2. 10th ed. London: Hodder Arnold. p.1474-1507.
NCCLS. (National Committee of Clinical Laboratory Standard). (1997). Performance standard for antimicrobial disk susceptibility test, 6th ed. Approved Standard Documents M2. National Committee of Clinical Laboratory Standard, Wayne. PA, USA. 24-30p.
Pegues D.A., Ohl M.E., Miller S.I. (2002). Salmonella, including Salmonella typhi. In: Blaser MJ, Smith PD, Ravdin JI, Greenberg HB, Guerran RL (eds.). Infections of the gastrointestinal tract. 2nd ed. Philadelphia: Lippincott Williams & Wilkins. 669-697p.
PIMA (Programa Integral de Mercado Agropecuario). (2013). Tendencias del consumo de frutas, hortalizas y pescado en las familias de Costa Rica. Costa Rica. 35-45p.
Prescott L., Harley J., Klein D. (2004). Microbiología. Quinta edición. McGraw Hill Interamericana. Madrid,
España. 145-162p.
Pouch F., Ito K. (2001). Compendium of methods for the microbiological examination of foods. Fourth edition. American Public Health Association. WA, USA. 83-100p.
Rodríguez E., Coronado M., H F., GarciaJ. (2005). Bacteriología general: Principios y prácticas de laboratorio. Editorial Universidad de Costa Rica. Costa Rica. 134, 213, 217, 265p.
Sacsaquispe R., Velásquez J. (2002). Manual de procedimientos para la prueba de sensibilidad antimicrobiana por el método de difusión. Serie de normas técnicas N. 30. Instituto nacional de salud. Lima, PER.
Saldar A., Armstrong D. (2003). Antimicrobial activities against 84 Listeria monocytogenes isolates from patients with sistemic listeriosis at a comprensive cancer center (1955-1997). J Clinical Microbiol. 49:483-485.
Sayah R., Kaneene J., Johnson Y., Miller R. (2005). Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic and wild animal fecal samples, human sewage and surface water. Appl
Environ Microbiol. 71:1394-1404.
Schroeder B.K., Du Toit L.J., Schwartz H.F. (2009). First report of Enterobacter cloacae causing onion bulb rot in the Columbia Basin of Washington State. Plant Dis. 93: 323.
Schwartz H.F., Gent D. (2007). Onion soft rot. High Plains IPM Guide, a cooperative effort of the University of Wyoming, University of Nebraska, Colorado State University and Montana State University, USA.
Schwartz H.F., Mohan S.K. (2008). Compendium of onion and garlic diseases and pests, 2nd ed. American Phytopathological Society (APS Press), St. Paul, MN, USA.
Schwartz H.F., Otto K. (2000). First report of a leaf and bulb decay of onion by Pantoea ananatis in Colorado.
Plant Dis. 84:808.
SEPSA. Secretaría Ejecutiva de Planificación Sectorial Agropecuaria (SEPSA). 2014. Boletín Estadístico
Agropecuario Nº 25. Costa Rica. 17-40.
SFE. Servicio Fitosanitario del Estado (SFE). 2015. https://www.sfe.go.cr/insumosys/Principal.html. Consultado el 29 de agosto de 2015.
Silliker J.H., Gabis D.A. (1976). ICMSF methods studies. VII indicator tests as substitutes for direct testing
of dried foods and feeds for Salmonella. Can. J. Microbiol. 22:971.
Stockwell V.O., Duffy B. (2012). Use of antibiotics in plant agriculture. Rev. sci. tech.Off. int. Epiz., 31: 199-210
Tauxe R., Kruse H., Hedberg C. (1997). Microbial hazards and emerging issues associated with produce, a preliminary reportto the national advisory committee on microbiologic criteriafor foods. J Food Prot. 60:1400-1408.
Uzeh R.E., Alade F.A., Bankole M. (2009). The microbial quality of pre-packed mixed vegetable salad in some retail outlets in Lagos Nigeria. Africal Journal of Food Science. 3: 270-272.
Vavilov N.I. (1951). The origin, variation, immunity and breeding of cultivated plants. Chronicles of botany
3: 1.
Vidaver A.K. (2002). Uses of antimicrobials in plant agriculture. Clin. Infect. Dis. 34:107-110.
Walcott R.R., Gitaitis R.D., Castro A.C., Sanders F.H., Díaz-Pérez J.C. (2002). Natural infestation of onion seed by Pantoea ananatis, causal agent of center rot. Plant Dis 86:106-111.
Wang A. (2013). Enfermedades bacterianas de la cebolla. In: M. Granaddos (ed). Problemas fitosanitarios de
la cebolla en Costa Rica. Primera edición. Facultad de Ciencias Agroalimentarias,Universidad de Costa Rica, San José, Costa Rica.
Zaid A.M.A., Bonasera J.M., Beer S.V. (2011). First report of Enterobacter decay caused by Enterobacter cloacae in New York. Plant Dis. 95:1581.
Publicado
2018-11-15
Cómo citar
Quesada, A. ., Chin, J. S. ., Aguilar, P. ., & Ruiz, K. . (2018). Sensibilidad a los antibióticos en bacterias Gram negativas de bulbos de cebolla en Tierra Blanca de Cartago. Repertorio Científico, 20(2), 121–130. https://doi.org/10.22458/rc.v20i2.2395
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