@article{Rodríguez-Núñez_Toledo_Arias_2016, title={Isolation of two diatom species with aquaculture potential (Bacillariophyceae) from the Pacific of Costa Rica}, volume={8}, url={https://revistas.uned.ac.cr/index.php/cuadernos/article/view/1228}, DOI={10.22458/urj.v8i1.1228}, abstractNote={<p class="x06-1-Abstract-y-Key-words" xml:lang="en-US"><span class="hps">D</span>ue to the importance of primary production in the trophic network, most aquatic ecosystems rely on microalgae. However, there are fewer studies on <span class="hps">phytoplankton</span> in the Central American region, specifically on marine diatoms, in part because it is difficult <span class="hps">to isolate and cultivate local species in tropical climates. The objective of this study is the isolation of two local diatoms </span>of <span class="char-style-override-4">Chaetoceros</span> and <span class="char-style-override-4">Nitzschia</span>, potential food for aquatic organisms. W<span class="hps">ater samples was taken from the Nicoya Gulf, located in the Central Pacific coast of</span> Costa Rica. The samples were successively filtered first through a 35µm pore size filter and then through one of 20µm. They were repeatedly isolated using modified Pasteur pipettes, and serial dilutions were done until a monoalgal culture was obtained. Two Bacillariophyceae were isolated: NGN1, genus <span class="char-style-override-4">Nitzschia;</span> and CGN2, genus <span class="char-style-override-4">Chaetoceros</span>. This study is a step toward reducing the importation of phytoplankton on feeding aquatic organisms by providing <span class="hps">technical and scientific information</span> <span class="hps">to develop a microalgal culture from this region. </span></p><p class="x06-1-Abstract-y-Key-words" xml:lang="en-US"><span class="hps"> </span></p>}, number={1}, journal={UNED Research Journal}, author={Rodríguez-Núñez, Karen and Toledo, Pedro and Arias, Sidey}, year={2016}, month={Jun.}, pages={93–98} }